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  • Mechanism of proliferating cell nuclear antigen clamp

    Apr 11, 2006 · A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. Sakato M, O'Donnell M, Hingorani MM. J Mol Biol, 416(2):163-175, 13 Dec 2011 Cited by: 16 articles | PMID: 22197374 | PMCID: PMC3269524. Free to read

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  • 10.1016/S0022-2836(12)00044-7 | DeepDyve

    Jun 11, 2020 · Abstract. Volume 416, Issue 2 Contents Articles A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA ATP Binding and Hydrolysis-Driven RateDetermining Events in the RFC-Catalyzed PCNA Clamp Loading Reaction Energy Complexes Are Apparently Associated with the Switchâ Motor Complex of Bacterial Flagella Molecular Structure and …

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  • (PDF) A structure-function analysis of the yeast Elg1

    The sliding clamp, PCNA, plays a central role in DNA replication and repair. In the moving replication fork, PCNA is present at the leading strand and at each of the Okazaki fragments that are formed on the lagging strand. PCNA enhances the

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  • Linchpin DNA-binding residues serve as go/no-go controls

    Moreover, their role depends on the clamp loader subunit; thus, Arg-84, Arg-88, and Arg-101 in RFC-B, RFC-C, and RFC-D, respectively, are increasingly important for stabilization of open PCNA upon ATP binding to RFC; Arg-88 in RFC-C is important for efficient positioning of ptDNA in the complex, which in turn enables further PCNA opening, ATP

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  • Role of PCNA and RFC in promoting Mus81-complex activity

    actively loaded onto DNA. This process requires a clamp loader, replication factor C (RFC), which loads PCNA onto primer-template junctions in an ATP-dependent manner [29, 30]. RFC is a heteropentameric complex composed of a large Rfc1 subunit (94.9 kDa) and four small subunits – Rfc2 (39.7 kDa), Rfc3 (38.2 kDa), Rfc4 (36 kDa), and Rfc5 (40 kDa).

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  • Journal of Molecular Biology | Vol 416, Issue 2, Pages 163

    Research article Full text access A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA

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  • Temporal Correlation of DNA Binding, ATP Hydrolysis, and

    Clamp loaders are multisubunit complexes that use the energy derived from ATP binding and hydrolysis to assemble ring-shaped sliding clamps onto DNA. Sliding clamps in turn tether DNA polymerases to the templates being copied to increase the processivity of DNA synthesis. Here, the rate of clamp release during the clamp loading reaction was measured directly for the first time using a FRET

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  • Mapping the interaction of DNA with the Escherichia coli

    Jan 16, 2005 · The RFC–PCNA complex suggested to us a mechanism for the recognition of target DNA by the ATP-loaded clamp loader 19. In the RFC–PCNA …

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  • Exploration of Prognostic Biomarkers among Replication

    Feb 12, 2021 · A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. J Mol Biol. 2012 ;416: 163 - 175 . Google Scholar | Crossref | Medline

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  • Structure of the human clamp loader reveals an

    Sep 09, 2020 · DNA replication and repair depend on AAA+ ATPase protein complexes called clamp loaders that open and load ring-shaped sliding clamps onto DNA. Using cryogenic electron microscopy, we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA). We assign this to be a reaction intermediate …

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  • A Central Swivel Point in the RFC Clamp Loader …

    however, pre-steady-state analysis provides a different view. The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP bindingtothissubunitinitiatesRFCactivation,andtheclamploaderadopts a spiral conformation that stabilizes PCNA in a corresponding open spiral.

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  • (PDF) Mechanism of PCNA Loading by RFC

    The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP binding to this subunit initiates RFC activation, and the clamp loader adopts a spiral conformation that

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  • Multifaceted regulation and functions of replication

    Aug 01, 2018 · A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. J Mol Biol. 2012; 416 :163–175. [ PMC free article ] [ PubMed ] [ Google Scholar ]

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  • The RFC Clamp Loader: Structure and Function

    Scheme of clamp loader function. (a) The structures of the E. coli β and human PCNA sliding clamps (PDB: 2POL and PDB: AXC, respectively).(b) Schematic of clamp loader function using eukaryotic RFC and PCNA as the example.ATP binding to RFC enables RFC to bind and open PCNA. In the presence of a primed template, RFC places PCNA onto DNA and then hydrolyzes ATP to eject from the PCNA-DNA

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    China Construction Machinery Business Network | To introduce you to this is my engine @ Weichai WP10-Weichai Yaxing (Yangzhou) New Energy Vehicle Co. Ltd-Weichai WP10 engine in the domestic loaders, is an indispensable existence. If you let every loader manufacturer such as a star on the micro-Bo on the general public, "Declaration of Love," most compa

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  • Michael E. O'Donnell - Publications

    A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. Journal of Molecular Biology. 416: 163-75. PMID 22197374 DOI: 10.1016/j.jmb.2011.12.017 : 1: 2012: Thompson JA, Marzahn MR, O'Donnell M, Bloom LB. Replication factor C is a more effective proliferating cell nuclear antigen (PCNA) opener than the checkpoint

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  • (PDF) Mechanism of PCNA Loading by RFC

    The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP binding to this subunit initiates RFC activation, and the clamp loader adopts a spiral conformation that

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