Apr 11, 2006 · A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. Sakato M, O'Donnell M, Hingorani MM. J Mol Biol, 416(2):163-175, 13 Dec 2011 Cited by: 16 articles | PMID: 22197374 | PMCID: PMC3269524. Free to read
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Jun 11, 2020 · Abstract. Volume 416, Issue 2 Contents Articles A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA ATP Binding and Hydrolysis-Driven RateDetermining Events in the RFC-Catalyzed PCNA Clamp Loading Reaction Energy Complexes Are Apparently Associated with the Switchâ Motor Complex of Bacterial Flagella Molecular Structure and …
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The sliding clamp, PCNA, plays a central role in DNA replication and repair. In the moving replication fork, PCNA is present at the leading strand and at each of the Okazaki fragments that are formed on the lagging strand. PCNA enhances the
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Moreover, their role depends on the clamp loader subunit; thus, Arg-84, Arg-88, and Arg-101 in RFC-B, RFC-C, and RFC-D, respectively, are increasingly important for stabilization of open PCNA upon ATP binding to RFC; Arg-88 in RFC-C is important for efficient positioning of ptDNA in the complex, which in turn enables further PCNA opening, ATP
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actively loaded onto DNA. This process requires a clamp loader, replication factor C (RFC), which loads PCNA onto primer-template junctions in an ATP-dependent manner [29, 30]. RFC is a heteropentameric complex composed of a large Rfc1 subunit (94.9 kDa) and four small subunits – Rfc2 (39.7 kDa), Rfc3 (38.2 kDa), Rfc4 (36 kDa), and Rfc5 (40 kDa).
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Research article Full text access A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA
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Clamp loaders are multisubunit complexes that use the energy derived from ATP binding and hydrolysis to assemble ring-shaped sliding clamps onto DNA. Sliding clamps in turn tether DNA polymerases to the templates being copied to increase the processivity of DNA synthesis. Here, the rate of clamp release during the clamp loading reaction was measured directly for the first time using a FRET
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Jan 16, 2005 · The RFC–PCNA complex suggested to us a mechanism for the recognition of target DNA by the ATP-loaded clamp loader 19. In the RFC–PCNA …
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Feb 12, 2021 · A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. J Mol Biol. 2012 ;416: 163 - 175 . Google Scholar | Crossref | Medline
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Sep 09, 2020 · DNA replication and repair depend on AAA+ ATPase protein complexes called clamp loaders that open and load ring-shaped sliding clamps onto DNA. Using cryogenic electron microscopy, we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA). We assign this to be a reaction intermediate …
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however, pre-steady-state analysis provides a different view. The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP bindingtothissubunitinitiatesRFCactivation,andtheclamploaderadopts a spiral conformation that stabilizes PCNA in a corresponding open spiral.
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The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP binding to this subunit initiates RFC activation, and the clamp loader adopts a spiral conformation that
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Aug 01, 2018 · A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. J Mol Biol. 2012; 416 :163–175. [ PMC free article ] [ PubMed ] [ Google Scholar ]
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Scheme of clamp loader function. (a) The structures of the E. coli β and human PCNA sliding clamps (PDB: 2POL and PDB: AXC, respectively).(b) Schematic of clamp loader function using eukaryotic RFC and PCNA as the example.ATP binding to RFC enables RFC to bind and open PCNA. In the presence of a primed template, RFC places PCNA onto DNA and then hydrolyzes ATP to eject from the PCNA-DNA
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A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA. Journal of Molecular Biology. 416: 163-75. PMID 22197374 DOI: 10.1016/j.jmb.2011.12.017 : 1: 2012: Thompson JA, Marzahn MR, O'Donnell M, Bloom LB. Replication factor C is a more effective proliferating cell nuclear antigen (PCNA) opener than the checkpoint
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The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP binding to this subunit initiates RFC activation, and the clamp loader adopts a spiral conformation that
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